Hepatitis C Viral RNA Genotype 3 NS5a Drug Resistance

Clinical Significance: For detection of mutations in the genotype 3 NS5a gene associated with resistance to NS5a inhibitors (example: daclatasvir, velpatasvir) including the Y93H polymorphism.

IDSA HCV Resistance Guidelines

Note: HCV Genotype 3 is required for this test. For HCV Genotype 1 NS5a Drug Resistance testing, refer to test guide #629.

6 mL blood in LAVENDER TOP (EDTA) tube (preferred)

Also Accepted: 6 mL blood in a PEARL TOP (PPT Potassium EDTA), RED TOP tube, or GOLD TOP (SST)

Centrifuge sample and transfer plasma/serum to a separate, SCREW-CAPPED plastic vial within 24 hours of collection. Refrigerate sample.

Login: SEND1-;REFRIG

• RSNDT1: QSTID
• RSTYP1: PLS (or SERUM)
• RTSRQ1:;HCV RNA Genotype 3 NS5a Drug Resistance (Quest Test 93325)

Sendouts:

• Review the order or contact the provider as needed to verify the genotype.
• For HCV Genotype 3 NS5a Drug Resistance testing, order Quest Test: 93325

Stability: Refrigerated (preferred): 14 days; Frozen: 42 days; Ambient: 72 hours.

Reject due to:

• Gross hemolysis
• Gross lipemia
• Specimens collected in heparin anticoagulant