Heme Single Gene by NGS

General Information

Lab Name

Lab Code

HOTSGH

Epic Ordering

Heme Single Gene Sequencing

Description

The Heme Single Gene by NGS (HOTSGH) assay is intended to test a single gene selected from the Heme Amplicon panel. See synonyms field above for the list of offered genes. The regions of the genes included are amplified using amplification technology and sequenced on an Illumina instrument to detect single nucleotide variants, insertions, and deletions. This test is designed to detect somatic mutations in the setting of neoplasia, and is not designed to detect germline (heritable) mutations.

Synonyms

11-17, 14, 15, 16), 17), 26, 27), 3, 6), 6), ASXL1 (CDS), CBL (CDS), CSF3R (Exons 14, DNMT3A (CDS), EZH2 (Exons 15-20), FBXW7 (CDS), FGFR1 (Exons 4, FLT3 (p.D835H), GATA1 (Exons 2-3), GATA2 (Exons 3-5), Hotspot, HRAS (Exon 1-2), IDH1( p.R132), IDH2 (Exon 4), JAK2 (Exon 12, KIT (8-18), KMT2A (CDS), KRAS (CDS), MAP2K1 (Exons 2, MPL (Exon 10), MYD88 (Exon 3-5), next-generation sequencing, NGS, NOTCH1 (Exons 20, NPM1 (Exon 12), NRAS (CDS), partial 18), PDGFRA (Exons 12-18), PHF6 (CDS), PTEN (CDS), RB1 (CDS), RUNX1 (Exon 4-8), SF3B1 (Exon 14-16), single gene, SRSF2 (Exon 1), STAG2 (CDS), STAT3 (Exons 20-21), TET2 (CDS), TP53 (CDS), U2AF1 (Exons 2, WT1 (CDS), ZRSR2 (CDS)

Components

Code	Name
SGHSD	Heme Gene Specimen Description
SGHRES	Heme Gene Result
SGHINT	Heme Gene Interpretation
SGHM	Heme Gene Method

Interpretation

Method

Amplification of the stated genetic regions followed by sequencing using an Illumina instrument

Reference Range

See individual components

Guidelines

Molecular Testing for Hematopoietic Disorders

Ordering & Collection

Specimen Type: Blood, Bone Marrow, Formalin-Fixed Paraffin-embedded Tissue (FFPE)
Collection: Blood: 6 mL blood in LAVENDER top (EDTA) tubeBone Marrow: 1-2 mL in a LAVENDER top (EDTA) tube
Handling Instructions: Samples should be refrigerated and delivered to the Laboratory without delay.

Extracted DNA will be accepted only from CLIA certified laboratories.
Quantity: requested: Entire tube

Processing

Processing: Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood

Samples submitted outside of these ranges will be evaluated for quality using appropriate internal controls and the results qualified appropriately.

Performance

LIS Dept Code

Molecular Hemepath- OLD (MHPO)

Performing Location(s)

Other	Hematopathology, Molecular 206-606-7060 UW Hematopathology Laboratory, G7-800 Fred Hutchinson Cancer Center 825 Eastlake Avenue E. Seattle, WA 98109	Hematopathology, Molecular hours: 7:00 am - 5:30 pm M-F 8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Frequency

setup weekly, results in 10-12 days.

Available STAT?

Billing & Coding

CPT codes
Billing Comments: For additional test/billing information, see Heme Single Gene by NGS CPT codes.
LOINC: 35474-6
Interfaced Order Code: UOW3081