Heme Amplicon Panel by NGS

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General Information

Lab Name
Heme Amplicon Panel by NGS
Lab Code
HOTSPT
Epic Ordering
Heme Amplicon Panel by NGS
Description

UW Heme Amplicon panel is a multiplexed gene panel assay that detects mutations related to treatment, prognosis, or diagnosis in the setting of hematolgical malignancy. The regions of the genes included in the panel are amplified using amplification technology and sequenced on an Illumina instrument to detect single nucleotide variants, insertions, and deletions. This test is designed to detect somatic mutations in the setting of neoplasia, and is not designed to detect germline (heritable) mutations.

Synonyms
11-17, 14, 15, 16), 17), 26, 27), 3, 6), 6), amplicon panel, ASXL1 (CDS), CBL (CDS), CSF3R (Exons 14, DNMT3A (CDS), EZH2 (Exons 15-20), FBXW7 (CDS), FGFR1 (Exons 4, FLT3 (p.D835H), GATA1 (Exons 2-3), GATA2 (Exons 3-5), Hotspot, HRAS (Exon 1-2), IDH1( p.R132), IDH2 (Exon 4), JAK2 (Exon 12, KIT (8-18), KMT2A (CDS), KRAS (CDS), MAP2K1 (Exons 2, MPL (Exon 10), multigene panel, MYD88 (Exon 3-5), next-generation sequencing, NGS, NOTCH1 (Exons 20, NPM1 (Exon 12), NRAS (CDS), partial 18), PDGFRA (Exons 12-18), PHF6 (CDS), PTEN (CDS), RB1 (CDS), RUNX1 (Exon 4-8), SF3B1 (Exon 14-16), SRSF2 (Exon 1), STAG2 (CDS), STAT3 (Exons 20-21), TET2 (CDS), TP53 (CDS), U2AF1 (Exons 2, WT1 (CDS), ZRSR2 (CDS)
Components

Interpretation

Method

Amplification of the stated genetic regions followed by sequencing using an Illumina instrument

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Blood, Bone Marrow, Formalin-Fixed Paraffin-embedded Tissue (FFPE)
Collection

Blood: 6 mL blood in LAVENDER top (EDTA) tubeBone Marrow: 1-2 mL in a LAVENDER top (EDTA) tube

Handling Instructions

Samples should be refrigerated and delivered to the Laboratory without delay.

Extracted DNA will be accepted only from CLIA certified laboratories.

Quantity
requested: Entire tube

Processing

Processing

Optimal results are achieved when samples are received within: 72 hours of collection for bone marrow and peripheral blood

Samples submitted outside of these ranges will be evaluated for quality using appropriate internal controls and the results qualified appropriately.

Performance

LIS Dept Code
Molecular Hemepath- OLD (MHPO)
Performing Location(s)
Other Hematopathology, Molecular
206-606-7060

UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109

Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays
Frequency
setup weekly, results in 10-12 days.
Available STAT?
No

Billing & Coding

CPT codes
LOINC
35474-6
Interfaced Order Code
UOW3087