BCR/ABL p210, Quant by RT PCR

General Information

Lab Name

Lab Code

QP210

Epic Ordering

BCR/ABL p210 Quantitative

Description

This assay is a nucleic acid amplification test for the quantitation of BCR::ABL1 p210 transcripts (e13a2 and e14a2) in total RNA from whole blood or bone marrow aspirate. This quantitative test is recommended for therapeutic monitoring and detection of minimal residual disease for patients with an established diagnosis. The test does not differentiate between e13a2/b2a2 or e14a2/b3a2 fusion transcripts and does not detect other rare fusion transcripts resulting from t(9;22).

To facilitate the interlaboratory comparison of findings and the assessment of molecular milestones (major molecular response or MMR), results are reported using the International Scale.

For the p190 fusion form (minor breakpoint), order BCR/ABL p190, Quant by RT PCR [QP190]

For patients with uncertain diagnosis or unknown forms of BCR::ABL1 fusion transcripts, please consider ordering BCR/ABL p210, Quant Reflexive [QP210R]
The reportable range is 0.0030% to 50% IS

The limit of detection is 0.0030% IS.

Stability is 72 hrs. Specimens received after 72 hrs. will be rejected.

Limitations: This assay in unable to detect BCR::ABL1 p190, p230, or other rare transcripts.

Please complete the appropriate request form based on your patient location. Hematopathology test request form can be found at Clinical Pathology Test Order Forms.

Forms & Requisitions

Requisition

Synonyms

ALL, B-ALL, B-Cell Acute Lymphoblastic Leukemia, BALL, BCR::ABL1, Chronic Myelogenous Leukemia, CML, p210, P210, Philadelphia chromosome Ph major, QP210, Quantitative BCR/ABL p210, Quantitative RT-PCR, t(9;22)

Components

Code	Name
P210SD	BCR/ABL p210 Specimen Desc
P210RS	BCR/ABL p210 Result
P210IS	BCR/ABL p210 %IS
P210IN	BCR/ABL p210 Interpretation
P210M	BCR/ABL p210 Method
P210GR	P210 Case Number

Interpretation

Method

RT-PCR

Reference Range

See individual components

Guidelines

Molecular Testing for Hematopoietic Disorders

Ordering & Collection

Specimen Type: Peripheral Blood or Bone Marrow Aspirate collected in EDTA.
Collection: Blood: 6mL in LAVENDER top (EDTA) (minimum total of 5 million WBC)

Bone Marrow: 1-2 mL in a LAVENDER top (EDTA) (minimum total of 5 million WBC)

Unacceptable:
Serum, plasma, extracted DNA, CSF, FFPE tissue, frozen whole blood or bone marrow.
Severely hemolyzed or clotted specimens.
Specimens with low volume or inadequate WBC.
Specimens received beyond 72hr stability limit.
Specimens collected in anticoagulants other than EDTA.
Forms & Requisitions: Requisition
Handling Instructions: Samples should be transported at RT and arrive in lab within 24 hours.
If sample is expected to arrive in the HP lab beyond 24 hours, store and transport at 2 – 6˚C.
The stability limit is 72 hours, samples beyond this limit will be rejected.
Quantity: requested: Blood: 6mL in EDTA, Bone Marrow: 2mL in EDTA
minimum: Blood: 4mL in EDTA, Bone Marrow: 1mL in EDTA

Processing

Processing: Send specimens to Hematopathology – Molecular department for processing.

Performance

LIS Dept Code

Molecular Hematopathology (MHP)

Performing Location(s)

Other	Hematopathology, Molecular 206-606-7060 UW Hematopathology Laboratory, G7-800 Fred Hutchinson Cancer Center 825 Eastlake Avenue E. Seattle, WA 98109	Hematopathology, Molecular hours: 7:00 am - 5:30 pm M-F 8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays

Frequency

Monday - Saturday. Results available within 5 days of receipt.

Available STAT?

Billing & Coding

CPT codes: 81206
LOINC: 21822-2
Interfaced Order Code: UOW2738