Procollagen I Intact N-Terminal (Sendout)

General Information

Lab Name

Lab Code

RPINP

Epic Name

Procollagen I Intact N-Term (Sendout)

External Test Id

PINP

Description

Useful For:

Aiding in monitoring antiresorptive and anabolic therapy in patients with osteoporosis
An adjunct in the assessment of conditions associated with increased bone turnover such as Paget disease
This test should not be used as a screening test for osteoporosis in the general population.

Ordering Guidance: This test should not be requested in patients who have recently received radioisotopes, therapeutically or diagnostically, because of potential assay interference. A recommended time period before collection cannot be made because it will depend on the isotope administered, the dose given and the clearance rate in the individual patient. Specimens will be screened for radioactivity prior to analysis. Radioactive specimens received in the laboratory will be held and assayed after the radioactivity has sufficiently decayed. This will result in a test delay.

Synonyms

P1NP, PINP, Procollagen I Intact N-Terminal, Procollagen Type 1 Intact N-Terminal Propeptide

Components

Code	Name
RPINPR	Procollagen I Intact N-Terminal

Interpretation

Method

Method Description: The procollagen I intact N-terminal (PINP) kit is based on the competitive radioimmunoassay technique. A known amount of labeled PINP and an unknown amount of unlabeled PINP in the sample compete for a limited number of high-affinity binding sites of the polyclonal rabbit anti-PINP antibody. A second antibody, directed against rabbit IgG and coated Kaolin particles, is used to separate the antibody-bound PINP from free PINP. The radioactivity of the bound tracer antigen is measured on a gamma counter. The amount of labeled PINP in the sample tube is inversely proportional to the amount of PINP in the sample. The concentrations in unknown samples are obtained from a calibration curve, which is based on the concurrent testing of PINP calibrators.(Package insert: UniQ PINP RIA, Intact N-terminal propeptide of type I procollagen. Orion Diagnostica; 03/2016)

Reference Range

See individual components

Ref. Range Notes

Reference Values:

Adult male:	22-87 mcg/L
Adult female premenopausal:	19-83 mcg/L
Adult female postmenopausal:	16-96 mcg/L

Reference values have not been established for patients who are <18 years of age.

Interferences and Limitations

Cautions:

There is diurnal variation of procollagen I intact N-terminal propeptide (PINP) levels, with the values being higher at night. When serial measurements of PINP are performed, specimens should be collected at the same time of the day.

PINP is metabolized in the liver. In individuals with severe liver disease, clearance from the circulation might be affected resulting in elevated PINP levels.

Some patients that have been exposed to mouse antigens, whether in the environment or as part of treatment or imaging procedures, may have circulating antimouse antibodies. These antibodies may interfere with the assay reagents to produce unreliable PINP assay results.

Ordering & Collection

Specimen Type: Blood
Collection: 3 mL blood in RED TOP Tube or GOLD SST
Handling Instructions: Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Freeze serum at -20°C while awaiting shipment. Transport on dry ice.

Stability: Frozen (Preferred): 180 days; Refrigerated: 7 days; Ambient: 7 days.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.
Quantity: Requested: 0.5 mL serum
Minimum: 0.25 mL serum

Processing

Receiving Instructions

Centrifuge sample and transfer serum to a separate plastic vial within two hours of collection. Freeze serum at -20°C.

Sendouts:

Order Mayo Test: PINP
Interfaced: Yes [Interface: 601; Worksheet: MAFZ]

Stability: Frozen (Preferred): 180 days; Refrigerated: 7 days; Ambient: 7 days.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus.

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (FZ)(MAFZ)

Frequency

Performed: Tuesday, Thursday. Report Available: 1-5 days.

Available STAT?

Performing Location(s)

Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901

Billing & Coding

CPT Codes: 83519
LOINC: 77370-5
Interfaced Order Code: UOW5835