Hepatitis C Viral RNA Genotype 3 NS5a Drug Resistance

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General Information

Lab Name
Lab Code
911
Description

Clinical Significance: For detection of mutations in the genotype 3 NS5a gene associated with resistance to NS5a inhibitors (example: daclatasvir, velpatasvir) including the Y93H polymorphism.

IDSA HCV Resistance Guidelines

Note: HCV Genotype 3 is required for this test. For HCV Genotype 1 NS5a Drug Resistance testing, refer to test guide #629.

Synonyms
Daclatasvir Resistance, HCV NS5a Subtype, Velpatasvir Resistance
Components

Interpretation

Method

Polymerase Chain Reaction (PCR), Sequencing

Reference Range

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in LAVENDER TOP (EDTA) tube (preferred)

Also Accepted: 6 mL blood in a PEARL TOP (PPT Potassium EDTA), RED TOP tube, or GOLD TOP (SST)

Quantity
Requested: 2 mL plasma/serum
Minimum: 0.6 mL plasma/serum

Processing

Receiving Instructions

Centrifuge sample and transfer plasma/serum to a separate, SCREW-CAPPED plastic vial within 24 hours of collection. Refrigerate sample.

Login: SEND1-;REFRIG

  • RSNDT1: QSTID
  • RSTYP1: PLS (or SERUM)
  • RTSRQ1:;HCV RNA Genotype 3 NS5a Drug Resistance (Quest Test 93325)

Sendouts:

  • Review the order or contact the provider as needed to verify the genotype.
  • For HCV Genotype 3 NS5a Drug Resistance testing, order Quest Test: 93325

Stability: Refrigerated (preferred): 14 days; Frozen: 42 days; Ambient: 72 hours.

Reject due to:

  • Gross hemolysis
  • Gross lipemia
  • Specimens collected in heparin anticoagulant
Misc Sendout

Performance

Lab Department
Frequency
Set up: Tues. Report available: 4-11 days.
Available STAT?
No
Performing Location(s)
Sendout Quest Diagnostics Infectious Diseases, Inc.
800-445-4032

33608 Ortega Highway
San Juan Capistrano, CA 92675

Billing & Coding

Billing Comments

CPT: 87902