Anti Endomysial, IgA (by IFA)

General Information

Lab Name: Anti Endomysial, IgA
Lab Code: AEMYA
Epic Ordering: Anti Endomysial, IgA
Description: The presence of IgA endomysial autoantibodies by indirect immunofluorescence is suggestive of active gluten sensitive enteropathies such as celiac disease. Results are primarily useful for diagnosis; they may also be used for monitoring response to treatment with a gluten-free diet, but results are semi-quantitative.
References: Bürgin-Wolff, et al., Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. (1991) 66:941.
Synonyms: Celiac, Celiac Ab, Endomysium, Sprue

Method

Indirect immunofluorescence (IFA) using INOVA monkey esophagus tissue to detect IgA autoantibodies.

Reference Range

Female		Male
Age	Range	Age	Range
0-	Negative [NRN]	0-	Negative [NRN]

Effective date: 04/01/2010

Ref. Range Notes

Established by testing healthy blood donors during method validation.

Interferences and Limitations

Anti-smooth muscle antibodies may obscure endomysial staining. The test cannot be used for patients with IgA deficiency.

Grossly hemolyzed or lipemic serum may produce increased nonspecific background staining of substrate.

References

Bürgin-Wolff, et al., Antigliadin and antiendomysium antibody determination for coeliac disease. Arch Dis Child. (1991) 66:941.

Guidelines

Specimen Type: Blood
Collection: 3 mL blood in RED TOP or SST tube
Handling Instructions: Instructions for Outside Clients: Freeze serum at -20°C

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term
Quantity: requested: 1 mL serum
minimum: 0.3 mL serum

Processing: Freeze serum at -20°C

Note: If Anti Endomysial is requested without specifying method (i.e. IFA or EIA), then order: AEMYA

Note2: AEMYA may be reflexively added on to CELRP orders if indicated.

Stability: Room Temperature: up to 8 hours; Refrigerated: 14 days; Frozen at -20°C: Long Term

LIS Dept Code

Immunology (IMM)

Performing Location(s)

UW-MT	Immunology 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Wednesday, Friday. Negative results same day. Positive results on next run.

Available STAT?