AST (Roche)

General Information

Guidelines

Method

Spectrophotometry

Reference Range

Units: U/L

Female		Male		Unknown
Age	Range	Age	Range	Age	Range
0-	0-35	0-	0-50	0-	0-35

Effective date: 01/12/2026

Specimen Type: Peripheral Blood
Collection: HMC and UWMC Onsite Locations
Preferred: 4 mL blood in LIME GREEN PST tube
Also Acceptable: 4 mL blood in Orange RST, Gold SST, Red Top, and Green Top tube
Pediatric: 1 full LIME Green Microtainer (Gold Acceptable)
Unacceptable: Gray Top

Offsite Clinic or Other Locations
Preferred: 4 mL blood in Gold SST tube
Also Acceptable: 4 mL blood in Orange RST, Gold SST, Red Top, and Green Top tube
Pediatric: 1 full LIME Green Microtainer (Gold Acceptable)
Unacceptable: Gray Top
Quantity: Requested: 1 mL Serum/Plasma
Minimum: 0.5 mL Serum/Plasma

Receiving Instructions: Refrigerate serum/plasma

Unacceptable: Hemolysis

Note: If ordered with Alanine Aminotransferase, see ALT and AST [ALST].

Lab Department

Chemistry, Automated Panels(CHA)

Frequency

Monday through Sunday

Available STAT?

Yes

Performing Location(s)

FHCC	FHCC Peninsula Laboratory 19917 7th Ave NE Ste 100, Poulsbo, WA 98370-6555	Phone: 360-842-5116 Fax: 360-842-5150 Hours of Operation: 8:00 a.m. - 5:00 p.m.
FHCC	Fred Hutch Alliance Lab 206-606-1088 825 Eastlake Ave, Seattle, WA 98109	Fax: 206-606-2027 Hours of Operation: 6:00 a.m. - 10:00 p.m. Includes satellite labs: • SAIM - Sloan Clinic 1, 6th Floor • FHH3 - Sloan Clinic 2, 3rd Floor • FHH4 - Sloan Clinic 2, 4th Floor
HMC	Chemistry, Automated 206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Chemistry, Automated 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195
UW-NW	Main Lab 206-668-1344 UW Medical Center – Northwest 1550 N 115th Street, A200 Seattle, WA 98133