CMV Quant PCR for Eye Fluids

General Information

Lab Name

CMV Quant PCR for Eye Fluids

Lab Code

CMVEYE

Epic Name

CMV Quantitative PCR, Eye Fluids

Description

The detection and quantitation of Cytomegalovirus (CMV) are performed using a modified FDA-approved Roche cobas® CMV Quantitative Assay, which allows for rapid identification through real-time PCR amplification. The lower limit of detection (LoD) and the lower limit of quantitation (LLoQ) are both 34.5 IU/mL. The LoD is defined as the lowest viral concentration that produces a positive result in 95 percent of replicates. The assay targets the UL54 gene using specific primers.

The performance characteristics of the modified assay were determined by the University of Washington Department of Laboratory Medicine and Pathology. This modified version has not been cleared or approved by the U.S. Food and Drug Administration.

For Blood, CSF or Clear non-cellular fluid (e.g. BAL or Washing) and Swab see CMV Quantitative by PCR [CMVQN].

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. Washing), see CMV Qualitative by PCR, Tissue/Cells [CMVQLT].

For Dried Blood Spot, see CMV Quant by PCR, Blood Spots [CMVBSQ].

Synonyms

CMV-Vitreous

Components

Code	Name
CMVQNS	CMV Quant Specimen Description
CMQNR	CMV Quant Result
CMLOG	CMV DNA IU/mL (Log10)
CMVQNI	CMV Quant Interpretation