Protein Electrophoresis Reflexive Panel

General Information

Lab Name

Protein Electrophoresis Panel

Lab Code

ELPP

Epic Ordering

Protein Electrophoresis Reflexive Panel

Description

Purpose: This test can be used to screen for and identify a monoclonal protein (M-spike).

The initial test performed is serum protein electrophoresis. Samples with a monoclonal band present on protein electrophoresis that has previously been identified in this laboratory will be resulted with the concentration of the known M-spike. Samples demonstrating previously unidentified protein bands of concern will be reflexively tested by immunofixation, which generates an additional result and charge. If a monoclonal band is identified by immunofixation, its concentration will be reported.

Serum free light chain testing (FLC) testing is available as a separate order, and is not included in this panel.

Synonyms

Gammopathy, Immunofixation, M-Protein, MGUS, Monoclonal, Monoclonal antibody, Multiple myeloma, Myeloma, Serum electrophoresis, Serum protein electrophoresis, SPEP, Waldenstrom

Components

Code	Name
ETP	Protein (Total)
EALB	Albumin
EA1	Alpha 1
EA2	Alpha 2
EB	Beta
EG	Gamma
INTRPE	Electrophoresis Interp:

Interpretation

Method

Agarose Electrophoresis (AEP)

Reference Range

See individual components

Interferences and Limitations

Hemolysis may falsely elevate alpha-2 and beta components.
Stability: 1 week refrigerated; stable long-term if frozen.

Guidelines

Specialty Testing in Immunology

Ordering & Collection

Specimen Type: Blood
Collection: 3 mL blood in RED TOP or GOLD SST tube.
Pediatric: 2 full GOLD or RED TOP MICROTAINERS.
Handling Instructions
Quantity: requested: 0.5 mL serum ELP; 0.5 mL for TP

Processing

Processing: Refrigerate serum.
Make two aliquots: 0.5 mL to IMM for ELP and 0.5 mL to CHEM for ETP.

OK to share ELP and IFIX on one tube if short sample. Forward shared pink slip to Immunology.

Outside Laboratory: If Total Protein is provided please free text "TP provided" after test ordered, and provide a copy of the slip to Panel Bench and Immunology.

Performance

LIS Dept Code

Immunology (IMM)

Performing Location(s)

UW-MT	Immunology 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Frequency

Monday-Friday

Available STAT?

Billing & Coding

CPT codes: 84165x2
LOINC: 24351-9
Interfaced Order Code: UOW2008