Methylguanine methyltransferase (MGMT) promoter methylation assay
General Information
- Lab Name
- MGMT Promoter Methylation, Somatic
- Lab Code
- MGMTMT
- Epic Ordering
- MGMT Promoter Methylation, Somatic
- Description
The methylguanine methyltransferase (MGMT) promoter methylation assay is useful in the evaluation of malignant gliomas; methylation of the MGMT promoter occurs in approximately 40% of malignant gliomas and is a predictive biomarker for response to alkylating chemotherapy. MGMT is a DNA repair protein that reverses the desired cytotoxic effects of alkylating chemotherapeutic agents. Many clinical trials have shown that patients with a hypermethylated MGMT promoter are more likely to benefit from temozolomide (TMZ) in addition to standard surgical and radiation therapy. MGMT promoter status has recently been incorporated into adjuvant therapy guidelines provided by the US National Comprehensive Cancer Network.
- Synonyms
- glioblastoma, glioma, hypermethylation, methylation, methylguanine methyltransferase, MGMT, O6-methylguanine-DNA methyltransferase, promoter, temozolomide
- Components
-
Code Name MGMTCH MGMT Promoter Methylation Clinical History MGMTRE MGMT Promoter Methylation Result MGMTIN MGMT Promoter Methylation Interpretation MGMETH MGMT Promoter Methylation Method MGMTDI MGMT Promoter Methylation Director
Interpretation
- Method
Tumor DNA is bisulfite converted and amplified with a PCR-based assay to assess MGMT promoter hypermethylation
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- FFPE, Tissue
- Collection
-
EITHER (a) slides OR (b) tissue block:
(a) Instructions for slide specimens: 1 slide at 4-micron thickness stained with hematoxylin-and-eosin AND 10 unstained, non-baked slides at 10-micron thickness (a minimum of 5 unstained slides is acceptable). Unstained slides can be on charged or uncharged slides.Note: sections should contain as much tumor as possible.
(b) Instructions for tissue block specimen: Provide complete tissue block containing tumor tissue. If there is more than one tissue block, please provide the block that has the greatest amount of tumor tissue. Tissue block will be returned at completion of testing.
(c) Add-on after prior testing in our lab: contact Genetics lab
Unacceptable: Decalcified sample
- Handling Instructions
Attach a copy of the pathology report for the tumor sample being submitted.
- Quantity
-
requested: Requested: Slides or tissue block as noted above.
minimum: Minimum: 5 unstained slides (10-micron thickness) with 20% tumor nuclei
Processing
- Processing
Hold slides or tissue blocks at room temperature.
Outside Laboratories: Ship at room temperature.
Performance
- LIS Dept Code
- Genetics (GEN)
- Performing Location(s)
-
UW-MT Genetics Attention: Genetics Lab
Clinical lab, Room NW220
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195Tel: 206-598–6429 M–F (7:30 AM–4:00 PM)
Fax: 206-616-4584
Lab email: cgateam@uw.eduTel (EXOME only): 206-543-0459
Faculty
Jillian Buchan, PhD, FACMG
Runjun Kumar, MD, PhD
Regina Kwon, MD, MPH
Christina Lockwood, PhD, DABCC, DABMGG
Abbye McEwen, MD, PhD
Colin Pritchard, MD, PhD
Vera Paulson, MD, PhD
Eric Konnick, MD, MS
He Fang, PhD - Frequency
- Run at least once a week; Typical Turnaround: 3 weeks *Turn around times may vary based on factors such as tissue acquisition and insurance preauthorization.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81287
- LOINC
- 60252-4
- Interfaced Order Code
- UOW5557