HLA Class I + II Typing (Sendout)

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General Information

Lab Name
HLA Class I + II Typing (Sendout)
Lab Code
RHL12T
Epic Name
HLA Class I + II Typing (Sendout)
External Test Id
3084-06
Description

Indications: Solid Organ Transplant.

    Test Information: This test uses molecular typing methods to identify alleles of the HLA A, B, C, DR and DQ genes. Assignment is at the low resolution level.

    References
    Forms & Requisitions
    Synonyms
    3084-06
    Components
    Code Name
    RHC1A1 HLA A (Allele 1)
    RHC1A2 HLA A (Allele 2)
    RHC1B1 HLA B (Allele 1)
    RHC1B2 HLA B (Allele 2)
    RHCBW1 HLA Bw (Allele 1)
    RHCBW2 HLA Bw (Allele 2)
    RHC1C1 HLA C (Allele 1)
    RHC1C2 HLA C (Allele 2)
    R1DRB1 DRB1 (Allele 1)
    R2DRB1 DRB1 (Allele 2)
    RDR345 DRB345 (Allele 1)
    R2D345 DRB345 (Allele 2)
    R1DQB1 DQB1 (Allele 1)
    R2DQB1 DQB1 (Allele 2)
    R1DQA1 DQA1 (Allele 1)
    R2DQA1 DQA1 (Allele 2)
    R1DPB1 DPB1 (Allele 1)
    R2DPB1 DPB1 (Allele 2)
    R1DPA1 DPA1 (Allele 1)
    R2DPA1 DPA1 (Allele 2)

    Interpretation

    Method

    Molecular

    Reference Range
    See individual components
    References

    Ordering & Collection

    Specimen Type
    Blood
    Collection

    10 mL blood in YELLOW TOP (ACD) tube

    Note: If concurrent ABO/Rh testing is ordered, the collection date and time must be written on the specimen labels.

    Forms & Requisitions
    Handling Instructions

    Outside Laboratories:

    • Store and transport whole blood at ambient temperature.
    • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

    Stability: Ambient

    Quantity
    Requested: 10 mL whole blood (ACD)
    Minimum: 7 mL whole blood (ACD)

    Processing

    Receiving Instructions

    Store and transport samples at ambient temperature.

      Sendouts (UW-MT/HMC) and FHCC SPS:

      • Bloodworks NW HLA Test: 3084-06
      • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
      • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
        • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
      • Send routine samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
      • STAT orders should be sent to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
        • STAT orders for this test are rare and require the provider to request approval from the HLA Lab. If approval is obtained, the HLA Lead Technical Coordinator will contact CSS to request expedited transport of a sample. CSS will create an HC1 task for SPS. If this is not done, orders should be considered routine.

      Stability: Ambient

      Misc Sendout

      Performance

      Lab Department
      Sendouts BWNW Lab (AB)(BWAB)
      Frequency
      Performed: Monday - Friday. TAT: 7 days.
      Available STAT?
      No
      Performing Location(s)
      Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
      206-689-6580

      921 Terry Ave
      6th Floor
      Seattle, WA 98104

      Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
      After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
      Fax: 206-689-6582

      Billing & Coding

      CPT Codes
      81370
      LOINC
      19146-0