HLA Final Crossmatch (Sendout)

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General Information

Lab Name
HLA Final Crossmatch (Sendout)
Lab Code
RHLAFC
Epic Name
HLA Final Crossmatch (Sendout)
External Test Id
0000-00HF
Description

Indications: Solid organ transplant.

    Test Information: Bloodworks NW placeholder code for final lymphocyte crossmatch for solid organ transplants. This test may be used for recipient (deceased or living donor) or donor (living donor) samples. Patient serum is crossmatched against donor lymphocytes to to detect antibodies against the donor, and against the patient's own lymphocytes to detect autoantibodies. The test performed varies by priority (3088-00 for routine lymphocyte crossmatch, 3088-02 for 24-hour turnaround time, and 3088-04 for STAT/4- to 8-hour turnaround with verbal result reporting). Donor results will file under the recipient.

    • Note: Test results may not be associated with this sample if final crossmatch testing is performed on a stored sample at Bloodworks Northwest.

      Additional Ordering Guidance: This test may be used as recipient "day of transplant" sample to meet CMS requirements.

        Ordering Requirements (STAT Orders Only):

        • Providers must notify Bloodworks Northwest HLA Laboratory of any STAT requests by calling (206) 817-7730. If this is not done, the lab will perform the test as routine.
        • Contact information is required for verbal result reporting. Include the name and phone/pager of the person receiving results in the Epic order.
        References
        Forms & Requisitions
        Synonyms
        0000-00HF, 3088-00, 3088-02, 3088-04
        Components
        Code Name
        RHLCOM HLA Antibody Order Status

        Interpretation

        Method

        Flow Cytometry technique

        Reference Range
        See individual components
        References

        Ordering & Collection

        Specimen Type
        Blood
        Collection

        3 x 10 mL Yellow Top (ACD) tubes and 2 x 10 mL Red top tubes

        Forms & Requisitions
        Handling Instructions

        Outside Laboratories: This test is for use solely with UW Medicine transplant protocols.

        Quantity
        Requested: 30 mL whole blood in Yellow ACD and 20 mL blood in Red Top (clot) tubes
        Minimum: 30 mL whole blood in Yellow ACD and 20 mL blood in Red Top (clot) tubes

        Processing

        Receiving Instructions

        Store and transport samples at ambient temperature.

          Sendouts (UW-MT/HMC) and FHCC SPS:

            • Bloodworks NW HLA Test: 0000-00HF
            • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
            • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
              • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
            • Send routine samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
            • STAT orders should be sent to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
              • *Providers must select "yes" at the Epic AOE question: "Is STAT testing required?" All other orders can be treated as routine.

            Stability: Ambient. Samples should be received at Bloodworks Northwest within 72 hours of collection.

            Misc Sendout

            Performance

            Lab Department
            Sendouts BWNW Lab (AB)(BWAB)
            Frequency
            Performed: As needed. Results Available (Varies by test selected): Routine: 3 business days [RLX]; 24-Hr TAT: 24 Hours [RLX24]; STAT: 4-8 hours [RLXS].
            Available STAT?
            Yes, only by arrangement with Bloodworks Northwest HLA Lab. Call (206) 689-6580 (or 206-817-7730 after hours) for approval.
            Performing Location(s)
            Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
            206-689-6580

            921 Terry Ave
            6th Floor
            Seattle, WA 98104

            Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
            After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
            Fax: 206-689-6582

            Billing & Coding

            Billing Comments

            Placeholder mnemonic without billing. See individual tests for CPT coding information.