Karius NGS Pathogen Detection (Sendout)

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General Information

Lab Name
Karius NGS Pathogen Detection (Sendout)
Lab Code
RKARUS
Epic Name
(not orderable in Epic)
External Test Id
Karius Spectrum™
Description

Ordering Information: Non-orderable sendout battery included in RKAPDG package.

Synonyms
Karius Spectrum
Components
Code Name
RKARUR Karius NGS Pathogen Detection Result
RKARPL Karius NGS Pathogen Detection Lab

Interpretation

Method

NGS

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood
Collection

Collect: 2 x 5 mL PEARL TOP PPT (preferred)

Also Accepted: 6 mL in LAVENDER K2-EDTA

Approval Required
Requires approval from Microbiology or Virology DLMP Faculty via the Laboratory Medicine Resident.
Quantity
Requested: 2 x 5 mL spun PPT tubes
Minimum: 0.7 mL plasma

Processing

Receiving Instructions

PEARL PPT: Centrifuge samples within 6 hours of collection. If only one pearl top was collected and sufficient sample is available, make an extra aliquot for test UW434 into a sterile plastic vial using sterile technique.

K2-EDTA: Centrifuge sample within 24 hours of collection and aliquot 0.7 mL plasma each into two sterile plastic vials using sterile technique (contact Micro lab for assistance). Do not disturb the buffy coat when transferring.

If there is insufficient plasma available, prioritize the sendout test.

  • RKARUS: Store samples in ambient Sendouts rack.
  • UW434: Forward to Molecular Micro for sample storage (-20°C).

If shipment is delayed: Samples >4 days old should be frozen at -20°C. (For PPT, freeze specimen in original collection tube without aliquotting.)

Sendouts:

  • Contact CAST for assistance with online ordering and requisition printing. Karius orders are considered urgent and should be expedited as much as possible.
    • CAST: The name and contact information of the ordering provider should be included in the order for direct result alerts.
  • Ship the sample the same day as collection (if possible) at ambient temperature. Wrap the sample with the provided gel pack to maintain temperature control. Do not use the enclosed shipping label for UPS pickup. Generate a separate FedEx label and select the "First Overnight" option.
  • Samples may be shipped on Friday for Saturday delivery.
  • Do not ship frozen samples in the Karius™ kit. Create a separate FedEx shipment label using the online FedEx account and ship with dry ice.

Stability: Ambient (preferred): 96 hours; Frozen: 6 months (spun, frozen PEARL PPT or EDTA plasma).

Misc Sendout

Performance

Lab Department
Serology Sendouts(SRSO)
Frequency
Performed: Monday - Sunday. TAT: Orders received by 08:30 a.m. cutoff are typically reported next day.
Available STAT?
No
Performing Location(s)
Sendout Karius, Inc.
866-452-7487

975 Island Drive
Suite 101
Redwood City, CA 94065

Billing & Coding

CPT Codes
0152U
LOINC
18725-2