Prospera (Sendout)

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General Information

Lab Name
Prospera Inhouse Collection
Lab Code
RPROSP
Epic Name
Prospera (Sendout) Panel (O367248)
External Test Id
Prospera™
Description

Test Information: Prospera™ uses single-nucleotide polymorphism (SNP)-based technology to assess for active rejection (AR) by measuring the DNA derived from transplanted donor organs (kidney, heart, lungs).

Ordering Requirements:

  • Providers must place an order for Prospera (Sendout) Panel (O367248) in Epic to ensure that order information and results transmit properly to Natera. This test code (RPROSP) is the collection component associated with the panel.
  • A Natera Prospera™ kit is preferred for testing, but not required. If patients will be drawn at a site that does not stock kits, providers may select the "Lab Collect - Send Kit" option in the Prospera Epic order to arrange for a kit to be shipped directly to the patient prior to collection.
Synonyms
dd cfDNA, Donor derived cell free DNA, Natera, Prospera Heart, Prospera Kidney, Prospera Lung, RPDDCD
Components
Code Name
RPRSPC Prospera Collection

Interpretation

Method

Single-nucleotide Polymorphism (SNP)

Reference Range
See individual components
Ref. Range Notes
dd-cfDNA Value: Interpretation:
≥1% Increased risk for active rejection
<1% Decreased risk for active rejection
Interferences and Limitations

Prospera is contraindicated:

  • Less than 24 hours after a biopsy
  • Less than 18 hours after dialysis
  • In pregnant women
  • In recipients of multi-organ or allogeneic cell transplants
  • In patients who have received an allograft from a genetically identical twin

Ordering & Collection

Specimen Type
Blood
Collection

Collect 20 mL blood in 2 x 10 mL Streck Cell-Free DNA BCT® tubes (mottled black and tan) from a Natera Prospera™ kit.

  • If Prospera™ kits are not available at the collection site, lab staff may use Streck tubes on hand. DO NOT COVER UP THE EXPIRATION DATE ON THE TUBE.
  • Fill all tubes completely and mix by gently inverting 8-10 times. Do not shake vigorously.
  • Walk samples to the laboratory after collection. Do not transport via pneumatic tube system, as this may compromise the Streck collection tubes.
  • When delivering samples to the lab, include the kit and the Natera requisition that prints from Epic.
  • Several backup kits may be stored in the lab, but clinics should not rely on DLMP to provide kits due to unknown volume of orders.
Handling Instructions

Outside Laboratories: Test available to UW Medicine patients only.

Quantity
Requested: 20 mL whole blood (2 full Streck tubes)
Minimum: 16 mL whole blood (2 Streck tubes with 8mL each)

Processing

Receiving Instructions

Store and transport whole blood at room temperature.

  • HMC and UW-NW: Forward samples and kit to UW-MT for sendout.
  • Fred Hutch: Send samples to Natera using provided kit. Close the order after sendout by entering NATSO for the pending RPROTR (Prospera Sample Tracking) test.

UW-MT Sendouts:

  • If samples have not been sourced from a Natera kit, ensure that the expiration date on the tube is visible.
  • Package samples along with the Natera requisition (printed from Epic) and ship at room temperature using the provided kit.
  • Samples may be shipped on Friday for Saturday delivery.

Stability: Ambient (required): 8 days; Refrigerated: Unacceptable; Frozen: Unacceptable.

CAST:

  • Print a pending UNATSO worksheet.
  • Use Sunquest 'Audit Trail' to confirm that the sample has been sent out.
  • Close the RPROSP order by entering NATSO for the pending RPROTR (Prospera Sample Tracking) component.
  • Results will file directly from Natera to Epic via interface.
Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
Turnaround Time: 3 days.
Available STAT?
No
Performing Location(s)
Sendout Natera

201 Industrial Road
San Carlos, CA 94070

Phone Numbers:

  • 650-273-4468 (Organ Health)
  • 844-778-4700 (Women's Health)
  • 650-489-9050 (Oncology)
Other Locations/Notes

Prospera Clinical Support (Organ Health):

  • Phone: 650.273.4468
  • Email: transplantclinical@natera.com

Billing & Coding

LOINC
30896-5
Billing Comments

No institutional billing. Patient self-pay or direct insurance billing only.