Prospera (Sendout)

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General Information

Lab Name
Prospera Inhouse Collection
Lab Code
RPROSP
Epic Name
Prospera (Sendout)
External Test Id
Prospera™
Description

Test Information: Prospera™ uses single-nucleotide polymorphism (SNP)-based technology to assess for active rejection (AR) by measuring the DNA derived from transplanted donor organs (kidney, heart, lungs).

    Ordering Requirements: A Natera Prospera™ kit is preferred for testing, but not required. If patients will be drawn at a site that does not stock kits, providers may select the "Lab Collect - Send Kit" option in the Prospera Epic order to arrange for a kit to be shipped directly to the patient prior to collection.

    Synonyms
    dd cfDNA, Donor derived cell free DNA, Natera, Prospera Heart, Prospera Kidney, Prospera Lung, RPDDCD
    Components
    Code Name
    RPRSPC Prospera Collection

    Interpretation

    Method

    Single-nucleotide Polymorphism (SNP)

    Reference Range
    See individual components
    Ref. Range Notes
    dd-cfDNA Value: Interpretation:
    ≥1% Increased risk for active rejection
    <1% Decreased risk for active rejection
    Interferences and Limitations

    Prospera is contraindicated:

    • Less than 24 hours after a biopsy
    • Less than 18 hours after dialysis
    • In pregnant women
    • In recipients of multi-organ or allogeneic cell transplants
    • In patients who have received an allograft from a genetically identical twin

    Ordering & Collection

    Specimen Type
    Blood
    Collection

    Collect 20 mL blood in 2 x 10 mL Streck Cell-Free DNA BCT® tubes (mottled black and tan) from a Natera Prospera™ kit.

    • If Prospera™ kits are not available at the collection site, lab staff may use Streck tubes on hand. DO NOT COVER UP THE EXPIRATION DATE ON THE TUBE.
    • Fill all tubes completely and mix by gently inverting 8-10 times. Do not shake vigorously.
    • Walk samples to the laboratory after collection. Do not transport via pneumatic tube system, as this may compromise the Streck collection tubes.
    • When delivering samples to the lab, include the kit and the Natera requisition that prints from Epic.
    • Several backup kits may be stored in the lab, but clinics should not rely on DLMP to provide kits due to unknown volume of orders.
    Handling Instructions

    Outside Laboratories: Test available to UW Medicine patients only.

    Quantity
    Requested: 20 mL whole blood (2 full Streck tubes)
    Minimum: 16 mL whole blood (2 Streck tubes with 8mL each)

    Processing

    Receiving Instructions

    Store and transport whole blood at room temperature.

    • HMC and UW-NW: Forward samples and kit to UW-MT for sendout.
    • Fred Hutch: Send samples to Natera using provided kit. Close the order after sendout by entering NATSO for the pending RPROTR (Prospera Sample Tracking) test.

    UW-MT Sendouts:

    • If samples have not been sourced from a Natera kit, ensure that the expiration date on the tube is visible.
    • Package samples along with the Epic requisition and ship at room temperature using the provided kit.
    • Samples may be shipped on Friday for Saturday delivery.

    Stability: Ambient (required): 8 days; Refrigerated: Unacceptable; Frozen: Unacceptable.

    CAST:

    • Print a pending UNATSO worksheet.
    • Use Sunquest 'Audit Trail' to confirm that the sample has been sent out.
    • Close the RPROSP order by entering NATSO for the pending RPROTR (Prospera Sample Tracking) component.
    • Results will file directly from Natera to Epic via interface.
    Misc Sendout

    Performance

    Lab Department
    Sendouts(SO)
    Frequency
    Turnaround Time: 3 days.
    Available STAT?
    No
    Performing Location(s)
    Sendout Natera

    201 Industrial Road
    San Carlos, CA 94070

    Phone Numbers:

    • 650-273-4468 (Organ Health)
    • 844-778-4700 (Women's Health)
    • 650-489-9050 (Oncology)
    Other Locations/Notes

    Prospera Clinical Support (Organ Health):

    • Phone: 650.273.4468
    • Email: transplantclinical@natera.com

    Billing & Coding

    LOINC
    30896-5
    Billing Comments

    No institutional billing. Patient self-pay or direct insurance billing only.