Flow Cytometry (Surface Marker Profile)

LAVENDER TOP (EDTA) or GREEN TOP (Heparin) are acceptable anticoagulants.

Specimen requirements and handling may vary depending on a number of clinical factors; therefore if there are questions or concerns, the laboratory should be consulted prior to obtaining the specimen.

Requirements and Storage

Blood

1-6mL LAVENDER TOP (EDTA) or GREEN TOP (HEPARIN) stored at ambient temp

Bone Marrow

First pull collected in LAVENDER TOP (EDTA) or GREEN TOP (HEPARIN) stored at ambient temp

Bone Marrow Biopsy

Submerged in RPMI media stored at 3-5C (REFRIGERATED)

Tissues

Submerged in RPMI media stored at 3-5C (REFRIGERATED)

CSF and BAL

Immediately stored 1:1 in RPMI media stored at 3-5C (REFRIGERATED)

Note: If the container is near full, add enough RPMI to still be able to safely store and/or ship.

Body Fluids

Stored at 3-5C (REFRIGERATED)

Rejection Criteria:

· Specimens received in the laboratory >7 days of collection for diagnostic or tissue samples, or >5 days for MRD testing

· Samples received frozen

· Specimen drawn into tubes containing anticoagulant different from specific requirements.

· Specimen drawn into tubes with separator gel, even if not spun

· Clotted (Note: Clotted sample will be disaggregated for testing. Data will be evaluated by Pathologist before sample is rejected.)

· Mislabeled or inadequately-labeled specimens

· Leaking/broken container with no specimen left inside container

· Quantity of sample not sufficient to perform test

· Tissues that have been held in a fixative such as formalin

· Severely hemolyzed specimens

· Since flow cytometry is based on evaluation of living cells, specimens should be received within 48 hours of collection for optimal viability. A disclaimer statement regarding limited assay sensitivity due to specimen age may be included in reports for specimens received >72 hours from collection.

· Specimens must be received in the laboratory within 7 days of collection for diagnostic or tissue samples, and 5 days for MRD testing. Specimens received beyond these criteria may be rejected upon receipt.

· The above packaging, transport, and shipping guidelines generally provide acceptable sample quality. However, it is at the discretion of the Pathologist to determine whether the resulting data is unacceptable or non-diagnostic.

SPS, log in as SAVHP and forward to Hematopathology Lab

Storage: BONE MARROW and BLOOD @ Room Temperature. CSF, BODY FLUIDS, and TISSUE in Refrigerator

Note: If specimen arrives after 6:30PM Monday-Sunday forward to Hematopath the following day.

Note2: Hematology/Hematopathology lab will add RPMI media to CSF or fluid specimens on receipt.