RhD Variant, Weak D Types 1,2,3 (Sendout)

Test Information: This test is useful for RhD typing issues that are probable weak D. For example, a pregnant woman who was serologically typed as RhD positive during first pregnancy and is currently typing as RhD negative.

Test Coverage: Weak D types 1, 2, & 3 only (no RHCE or other RHD variants).

Ordering Requirements:

• A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders. Leave the billing section blank to be completed by Sendouts staff.

Whole Blood: 10 mL whole blood in LAVENDER TOP (EDTA) tube

• Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at (206) 689-6269 for approval of microtainers.

Labeling Requirements:

• The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
• A matching draw date must be included on the specimen label and the requisition form.

Samples sent on Fridays must be received at Bloodworks Northwest Genomics Lab by 1 p.m.

Outside Laboratories:

• Store and transport whole blood or cultured amniocytes at ambient temperature.
• Expedite transport; samples must be received by Bloodworks NW within 48 hours of collection and before 1 p.m. on Fridays. Allow for one day of transport from UW-MT to Bloodworks Northwest.
• Submit a completed Bloodworks Northwest Genomics Testing Laboratory Requisition along with the DLMP Requisition.* Leave the billing section blank to be completed by Sendouts staff.
  • *Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

Stability: Ambient: 48 hours.

Store and transport whole blood at ambient temperature.

• If the sample is collected on a Friday, notify Sendouts immediately upon sample receipt to ensure delivery to Bloodworks NW by the 1 p.m. cutoff.

Sendouts:

• Order Bloodworks NW Test: 3117-09.
• A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders.
  • The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
  • A matching draw date must be included on the specimen label and the requisition form.
• Affix CAST "Billing" and "Results To" labels to the outgoing requisition.
• Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
• Send orders as received with the UW DLMP or Delivery Express courier.
• Samples may be sent Monday through Friday only. If sent on a Friday, samples must be in lab by 1 p.m.
• Ambient whole blood samples must arrive at Bloodworks within 48 hours of collection.*

Stability: Ambient: 48 hours.

*Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.