RhD Variant, Weak D Types 1,2,3 (Sendout)

General Information

Lab Name

Lab Code

RRDVWD

Epic Name

RhD Variant, Weak D Types 1,2,3 (Sendout)

External Test Id

3117-09

Description

Test Information: This test is useful for RhD typing issues that are probable weak D. For example, a pregnant woman who was serologically typed as RhD positive during first pregnancy and is currently typing as RhD negative.

Test Coverage: Weak D types 1, 2, & 3 only (no RHCE or other RHD variants).

Ordering Requirements:

A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders. Leave the billing section blank to be completed by lab staff.

References

Bloodworks Northwest Test Information: RhD Variant - Weak D Types 1, 2, 3

Forms & Requisitions

Bloodworks Northwest Genomics Testing Laboratory Requisition

Synonyms

3117-09, RBC Genotype, RBC Genotyping, Red Blood Cell Genotyping, Rh Genotyping

Components

Code	Name
RRDVR	RhD Variant, Weak D Types 1,2,3 Result
RRDVL	RhD Variant, Weak D Types 1,2,3 Performing Lab

Interpretation

Method

Polymerase Chain Reaction (PCR) Based

Reference Range

See individual components

Ref. Range Notes

See report.

Interferences and Limitations

Molecular testing to predict the red blood cell phenotype does not always reflect the phenotypic expression of the blood group antigen. This may occur in cases where the individual has genetic variation that silences or alters the blood group antigen expression that is not included in the target region of the test, and also may occur with RhD zygosity testing. Test results must be interpreted with knowledge of how known polymorphisms affect blood group antigen expression.

References

Bloodworks Northwest Test Information: RhD Variant - Weak D Types 1, 2, 3

Ordering & Collection

Specimen Type

Whole Blood, Cultured Amniocytes

Collection

Whole Blood: 10 mL whole blood in LAVENDER TOP (EDTA) tube

Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at (206) 689-6269 for approval of microtainers.

Cultured Amniocytes: 2 T25 Flasks cultured to confluency.

Amniotic fluid is not accepted.
An EDTA whole blood sample from the mother of the fetus must accompany cultured amniocyte orders.
Samples sent on Fridays must be received at Bloodworks Northwest Genomics Lab by 1 p.m.

Labeling Requirements:

The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
A matching draw date must be included on the specimen label and the requisition form.

Forms & Requisitions

Bloodworks Northwest Genomics Testing Laboratory Requisition

Handling Instructions

Outside Laboratories:

Store and transport whole blood at ambient temperature.
Expedite transport; blood samples must be received by Bloodworks NW within 96 hours of collection (48 hours preferred). Allow for one day of transport from UW-MT to Bloodworks Northwest.
- Unless the cells are cultured at UW-MT Cytogenetics and Genomics Lab, clients should arrange to send cultured amniocyte samples directly to Bloodworks NW due to the short stability window (24 hours).
Submit a completed Bloodworks Northwest Genomics Testing Laboratory Requisition along with the DLMP Requisition.** Leave the billing section blank to be completed by Sendouts staff.
- **Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

Stability:

Cultured Amniocytes: Ambient: 24 hours
Whole Blood: Ambient: 96 hours (48 hours preferred)

Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.

Quantity

Requested: 10 mL EDTA whole blood or two T-25 flasks of cultured amniocytes
Minimum: 5 mL EDTA whole blood or two T-25 flasks of cultured amniocytes

Processing

Receiving Instructions

Store and transport whole blood or cultured amniocytes at ambient temperature.

Cultured amniocytes should be sent out same-day; notify Sendout staff upon sample receipt. On Fridays, cultured cells must be received at Bloodworks Northwest by the 1 p.m. cutoff.

Sendouts (UW-MT/HMC) and FHCC SPS:

Order Bloodworks NW Test: 3117-09
A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders.
- The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
- A matching draw date must be included on the specimen label and the requisition form.
UW-MT/HMC Only: If the institution is not already indicated on the requisition, attach a CAST "Bill/Results To" label.
Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
Send samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
Ambient or refrigerated whole blood samples must arrive at Bloodworks within 96 hours of collection (48 hours is preferred).

Stability:

Cultured Amniocytes: Ambient: 24 hours
Whole Blood: Ambient: 96 hours (48 hours is preferred)

Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.

Misc Sendout

Performance

Lab Department

Sendouts(SO)

Frequency

Analytic time: 3 days

Available STAT?

Performing Location(s)

Sendout	Bloodworks Northwest Platelet Immunology/Genomics Laboratory (Central HPG) 921 Terry Ave 6th Floor - SDL Seattle, WA 98104	Genomics Testing Laboratory (for RBC genotyping): (206) 689-6269 Platelet Immunology Laboratory: (206) 689-6543 Laboratory staffed for questions Monday through Friday, 08:00 am to 04:30 pm.

Billing & Coding

CPT Codes: 81403
LOINC: 18717-9
Interfaced Order Code: UOW6373