RhD Variant, Weak D Types 1,2,3 (Sendout)

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General Information

Lab Name
RhD Variant, Weak D types 1,2,3
Lab Code
RRDVWD
Epic Name
RhD Variant, Weak D Types 1,2,3 (Sendout)
External Test Id
3117-09
Description

Test Information: This test is useful for RhD typing issues that are probable weak D. For example, a pregnant woman who was serologically typed as RhD positive during first pregnancy and is currently typing as RhD negative.

    Test Coverage: Weak D types 1, 2, & 3 only (no RHCE or other RHD variants).

      Ordering Requirements:

      References
      Forms & Requisitions
      Synonyms
      3117-09, RBC Genotype, RBC Genotyping, Red Blood Cell Genotyping, Rh Genotyping
      Components
      Code Name
      RRDVH RhD Var. Weak D typ 1,2,3, Hist
      RRDVR RhD Var. Weak D typ 1,2,3, Rslt
      RRDVI RhD Var. Weak D typ 1,2,3, Intrp
      RRDVC RhD Var. Weak D typ 1,2,3, Cmnt
      RRDVL RhD Var. Weak D typ 1,2,3, Lab

      Interpretation

      Method

      Polymerase Chain Reaction (PCR) Based

      Reference Range
      See individual components
      Ref. Range Notes

      See report.

      Interferences and Limitations

      Molecular testing to predict the red blood cell phenotype does not always reflect the phenotypic expression of the blood group antigen. This may occur in cases where the individual has genetic variation that silences or alters the blood group antigen expression that is not included in the target region of the test, and also may occur with RhD zygosity testing. Test results must be interpreted with knowledge of how known polymorphisms affect blood group antigen expression.

      References

      Ordering & Collection

      Specimen Type
      Whole Blood
      Collection

      Whole Blood: 10 mL whole blood in LAVENDER TOP (EDTA) tube

      • Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at (206) 689-6269 for approval of microtainers.

      Labeling Requirements:

      • The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
      • A matching draw date must be included on the specimen label and the requisition form.
      Forms & Requisitions
      Handling Instructions

      Outside Laboratories:

      • Store and transport whole blood or cultured amniocytes at ambient temperature.
      • Expedite transport; samples must be received by Bloodworks NW within 48 hours* of collection. Allow for one day of transport from UW-MT to Bloodworks Northwest.
      • Submit a completed Bloodworks Northwest Genomics Testing Laboratory Requisition along with the DLMP Requisition.** Leave the billing section blank to be completed by Sendouts staff.
        • **Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

      Stability: Ambient: 48 hours*

      *Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.

      Quantity
      Requested: 10 mL EDTA whole blood
      Minimum: 5 mL EDTA whole blood

      Processing

      Receiving Instructions

      Store and transport whole blood at ambient temperature.

      Sendouts:

      • Order Bloodworks NW Test: 3117-09
      • A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders.
        • The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
        • A matching draw date must be included on the specimen label and the requisition form.
      • UW-MT/HMC Only: If the institution is not already indicated on the requisition, attach a CAST "Bill/Results To" label.
      • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
      • Send samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
      • Ambient whole blood samples must arrive at Bloodworks within 48 hours* of collection.

      Stability: Ambient: 48 hours*

      *Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.

      Misc Sendout

      Performance

      Lab Department
      Transfusion Support(TS)
      Frequency
      Analytic time: 3 days
      Available STAT?
      No
      Performing Location(s)
      Sendout Bloodworks Northwest Platelet Immunology/Genomics Laboratory (Central HPG)

      921 Terry Ave
      6th Floor - SDL
      Seattle, WA 98104

      Genomics Testing Laboratory (for RBC genotyping): (206) 689-6269

      Platelet Immunology Laboratory: (206) 689-6543

      Laboratory staffed for questions Monday through Friday, 08:00 am to 04:30 pm.

      Billing & Coding

      CPT Codes
      81403
      LOINC
      18717-9