TRAC ID Kidney (Sendout)

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General Information

Lab Name
TRAC ID Kidney (Sendout)
Lab Code
RTRCID
Epic Name
TRAC ID Kidney (Sendout)
External Test Id
403683P
Description

Test Information: Eurofins TRAC ID (Transplant Rejection Allograft Check + Infectious Disease) kidney donor-derived cell-free DNA (dd-cfDNA) assay examines transplant recipient plasma to monitor the percentage of dd-cfDNA in transplant recipient plasma post-transplant using next generation sequencing. Eurofins TRAC ID utilizes whole genome sequencing (WGS) to assess dd-cfDNA. This approach enables the quantification of non-human (viral) DNA in addition to the assessment of donor vs. recipient DNA. In the identification and quantification of the viral DNA the following viruses can be assessed: Cytomegalovirus (CMV), BK Virus (BKV), Epstein-Barr Virus (EBV), Herpes Simplex Virus-1 (HSV-1), Herpes Simplex Virus-2 (HSV-2), Varicella Zoster Virus (VZV), Human Herpes Virus-6 (HHV-6), and Torque Teno Virus (TTV).

    Ordering Requirements:

    • Pre-approved for outpatient UW Medicine renal clinic locations only. Orders from other locations must be reviewed and approved by the Laboratory Medicine Resident on-call.
    • Providers must complete the Epic order questions with the following required information:
      • Date of transplant
      • Parent/Child Relationship (Yes/No)
    References
    Synonyms
    403683P, dd cfDNA, Eurofins, Post-Kidney Transplant Monitoring
    Components
    Code Name
    RTRACD TRAC dd cfDNA Result
    RTRCMV CMV Qualitative
    RCMVSQ CMV Semi Quantitative
    RTRBKV BKV Qualitative
    RBKVSQ BKV Semi Quantitative
    RTREBV EBV Qualitative
    REBVSQ EBV Semi Quantitative
    RTHSV1 HSV 1 Qualitative
    RHSV1Q HSV 1 Semi Quantitative
    RTHSV2 HSV 2 Qualitative
    RHSV2Q HSV 2 Semi Quantitative
    RTHHV6 HHV 6 Qualitative
    RHHV6Q HHV 6 Semi Quantitative
    RTRVZV VZV Qualitative
    RVZVSQ VZV Semi Quantitative
    RTRTTV TTV Qualitative
    RTTVSQ TTV Semi Quantitative
    RTRIDC TRAC ID Comment
    RTRIDL TRAC ID Performing Lab

    Interpretation

    Method

    Whole Genome Sequencing (WGS)

    Reference Range
    See individual components
    Ref. Range Notes

    Reference Ranges:

    dd-cfDNA: <0.70%
    CMV Qualitative: Not Detected
    CMV Semi-Quantitative: Not Detected
    BKV Qualitative: Not Detected
    BKV Semi-Quantitative: Not Detected
    EBV Qualitative: Not Detected
    EBV Semi-Quantitative: Not Detected
    HSV-1 Qualitative: Not Detected
    HSV-1 Semi-Quantitative: Not Detected
    HSV-2 Qualitative: Not Detected
    HSV-2 Semi-Quantitative: Not Detected
    HHV-6 Qualitative: Not Detected
    HHV-6 Semi-Quantitative: Not Detected
    VZV Qualitative: Not Detected
    VZV Semi-Quantitative: Not Detected
    TTV Qualitative: Detected
    TTV Semi-Quantitative: 6.00-8.00 log(gC/mL)
    References

    Ordering & Collection

    Specimen Type
    Blood
    Collection

    Collection Instructions:

    • Refer to Eurofins TRAC Kidney Collection Instructions.
    • Use a butterfly collection needle. For best results, a 21G or 22G needle is suggested.
    • Draw 10 mL whole blood in a Streck cell-free DNA BCT® collection tube (included in kit), filling the tube completely. If a kit is not provided, lab staff may use Streck tubes on hand.
    • Gently invert tube 8-10 times to mix.
    • Affix specimen label. Ensure that the labels do not obstruct the expiration date on the tube; it must be visible or the sample will be rejected.
    Handling Instructions

    Outside Laboratories: This test is available to UW Medicine providers only. If desired, clients should arrange for testing directly with Eurofins Transplant Genomics.

    Quantity
    Requested: 10 mL whole blood in Streck cell-free DNA BCT® tube
    Minimum: 10 mL whole blood in Streck cell-free DNA BCT® tube

    Processing

    Receiving Instructions

    Store and transport whole blood at ambient temperature.

    Sendouts:

    Stability: Ambient: 7 days; Refrigerated: Unacceptable; Frozen: Unacceptable.

    Reject Due To: Frozen whole blood, samples received >7 days after collection, specimen other than Streck Cell-Free DNA tube, patient identifiers on the collection tube do not match the requisition.

    Misc Sendout

    Performance

    Lab Department
    Sendouts(SO)
    Frequency
    Performed: Monday through Saturday. Turnaround Time: 4-6 days from sample receipt at Eurofins.
    Available STAT?
    No
    Performing Location(s)
    Sendout Eurofins Transplant Genomics, LLC
    844-878-4723

    18000 West 99th Street
    Suite 100
    Lenexa, KS 66219

    Fax: 888-224-3499
    Email: info@transplantgenomics.com

    Billing & Coding

    LOINC
    51991-8
    Interfaced Order Code
    UOW6103